An Iodo derivative of lamivudine was synthesized and a method was developed and validated by UV spectroscopy for its determination. The compound was purified by column chromatography. The derivative was confirmed by chemical tests followed by spectral characterization using UV, FTIR spectral data. The study was focused on the effect of amine group in biological activity. The developed method for its determination was simple and validated as per ICH guidelines. The derivative has shown λmax at 226 nm in UV method. The linearity concentration range for the derivative was found to be 10 - 50 μg/ml and co-relation coefficient was found to be 0.996.
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